Alpha-glucosidase inhibitors from leaves of Forsythia suspense in Henan province.
- Author:
Wenyi KANG
1
;
Jinmei WANG
;
Li ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: China; Enzyme Inhibitors; chemistry; isolation & purification; Forsythia; chemistry; Glycoside Hydrolase Inhibitors; Kinetics; Plant Extracts; chemistry; isolation & purification; Plant Leaves; chemistry; alpha-Glucosidases; chemistry
- From: China Journal of Chinese Materia Medica 2010;35(9):1156-1159
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe alpha-glucosidase inhibitory compounds were searched from Forsythia suspense.
METHODThe active compounds were isolated by the method of bioassay-guided in vitro and column chromatographic techniques. The structures of compounds were identified by MS and NMR spectroscopy. The inhibitory kinetics of the isolated compounds were investigated.
RESULTThe ethyl acetate extract showed the strongest inhibitory activity, and five active compounds were isolated from this extract. The IC50 value of compound 1-5 were all lower than that of acarbose as positive control. Compound 1, the mixture of 1, 2 and 4 all showed noncompetitive type model on alpha-glucosidase.
CONCLUSIONCompound 1-3 as the inhibitors of alpha-glucosidase were reported for the first time. Compound 3 was isolated from the genus for the first time.
