Study on sensitization and mechanism of CGA-BSA.
- Author:
Xiaowu HUANG
1
;
Hongbo LIAO
;
Ping LIU
;
Hui LIN
;
Liyan YUAN
;
Yuan HU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chlorogenic Acid; immunology; Drugs, Chinese Herbal; adverse effects; Guinea Pigs; Histamine Release; Hypersensitivity; blood; immunology; Immunoglobulin E; blood; Mast Cells; immunology; Random Allocation; Serum Albumin, Bovine; immunology
- From: China Journal of Chinese Materia Medica 2010;35(9):1181-1184
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the sensitization and mechanism of artificial antigen of chlorogenic acid (CGA-BSA).
METHODUsing intensive immunization to establish allergy animal model on guinea pig and preparing antiserum and tissue for further test. Using HE staining to observe pathology change of lungs, trachea, liver. Using passive mast cell (PMC) degranulation test to observe the immunogenicity of CGA-BSA and using ELISA to detect IgE and histamine in plasma.
RESULTThere established allergy animal model on guinea pig, which include a increase cell degranulation by a ratio (63.58 +/- 10.23)% in PMC test, increase of specific antibody IgE and increase of histamine in plasma after provocation by ELISA.
CONCLUSIONAllergen CGA-BSA could provoke allergenic response in guinea pig, and the allergic response belongs to type I allergy.
