Pharmacokinetics study on paeoniflorin in radix paeoniae alba extract by LC-MS.
- Author:
Tiandong BAO
1
;
Qing YANG
;
Yingfeng ZHANG
;
Yu DONG
;
Yujie LI
;
Xiaoxin ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Benzoates; administration & dosage; blood; pharmacokinetics; Bridged-Ring Compounds; administration & dosage; blood; pharmacokinetics; Chromatography, Liquid; methods; Glucosides; administration & dosage; blood; pharmacokinetics; Male; Mass Spectrometry; methods; Monoterpenes; Paeonia; chemistry; Plant Extracts; administration & dosage; blood; pharmacokinetics; Rats; Rats, Wistar
- From: China Journal of Chinese Materia Medica 2010;35(9):1193-1196
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a LC-MS method to determine paeoniflorin concentration in rats plasma. The method was applied to investigate pharmacokinetics of paeoniflorin in rats in vivo.
METHODBlood samples were collected at different time after oral administration of Radix Paeoniae Alba extract at doses of 0.2, 0.4, 0.8 g x kg(-1). The paeoniflorin concentration in plasma was determined by LC-MS method. Pharmacokinetic parameters were fitted by WinNonlin 5.1 software package.
RESULTThe linear range and the average recovery of paeoniflorin were 2.5-500 microg x L(-1) (r = 999 4) and more than 80% (n = 5) , respectively. The inner- and inter-days precision were both less than 15%. The T1/2 was similar. The relationship between dose and AUC showed good linearity.
CONCLUSIONThe method described in this report has high sensitivity and selectivity, and was suitable for pharmacokinetic study of paeoniflorin. The kinetic process of paeoniflorin in palsma showed two-compartment model after oral administration of Radix Paeoniae Alba extract at doses of 0.2, 0.4, 0.8 g x kg(-1) to rats.
