Determination of quercetin-3-O-alpha-L-arabinopyranoside in Periploca forrestii by RP-HPLC.
- Author:
Huaguo CHEN
1
;
Xin ZHOU
;
Guihong CAO
;
Xiuhai GAN
Author Information
1. Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001, China. chenhuaguo1981@163.com
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
analysis;
Glycosides;
analysis;
Periploca;
chemistry;
Quercetin;
analogs & derivatives;
analysis
- From:
China Journal of Chinese Materia Medica
2010;35(10):1284-1286
- CountryChina
- Language:Chinese
-
Abstract:
The aim of the paper was to develop a HPLC method for the quality control of Periploca forrestii Schltr. The 18 samples were analyzed on a Hypersil C18 column. The mobile phase was methanol-water (33:67) and the flow rate was 1 mL x min(-1). The detection wavelength was at 370 nm and column temperature was 25 degrees C. The linear relationship was good (r = 0.999 9) in the range of 0.204 4-2.044 microg for quercetin-3-O-alpha-L-arabinopyranoside. The average recovery was 97.78% (RSD 0.8%, n = 9). The contents of 18 samples varied from 0.171% to 0.264%. The method showed high precision, good repeatability and stability, so it can be used to assess the quality of P. forrestii.
