Protective activity of different concentration of tea polyphenols and its major compound EGCG against whole body irradiation-induced injury in mice.
- Author:
Shaolai GUO
1
;
Yuan HU
;
Ping LIU
;
Yuyu WANG
;
Daihong GUO
;
Dongxiao WANG
;
Hongho LIAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Camellia sinensis; chemistry; Catechin; administration & dosage; analogs & derivatives; Disease Models, Animal; Female; Flavonoids; administration & dosage; Humans; Male; Mice; Phenols; administration & dosage; Plant Extracts; administration & dosage; Polyphenols; Radiation Injuries; drug therapy; enzymology; prevention & control; Radiation-Protective Agents; administration & dosage; Random Allocation; Superoxide Dismutase; blood; Whole-Body Irradiation; adverse effects
- From: China Journal of Chinese Materia Medica 2010;35(10):1328-1331
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the different concentrate of tea polyphenols (TP) and its compound for irradiation-protection and investigate its mechanism.
METHODTo evaluate the radioprotective activity, mice were exposed to whole body gamma irradiation. TP 80 and TP 50 (50, 10 mg x kg(-1)) and its major constituent epigallocatechin gallate (EGCG) (50, 10 mg x kg(-1)) were administered after irradiation to examine its inhibition against irradiation-induced injury.
RESULTThis study indicate that in comparison with non-irradiated controls, irradiation resulted in a significant reduction the spleen index (spleen weight/body weight 100), haematological parameters (RBC, WBC and PLT), activity of superoxide dismutase (SOD), and increase of malondialdehde (MDA) level in 28 days. Oral administration of TP (50 mg x kg(-1)) shown the best effect on reducing the irradiation-induced injury on mice studied, and showed a protective effect against irradiation-induced haematological parameters (RBC, WBC and PLT), the spleen index and MDA level significant reduction, and antioxidase activity (SOD) decrease.
CONCLUSIONThe results suggest that TP 50 mg x kg(-1) and EGCG have in vivo antioxidant potential and radioprotective activity against whole body gamma irradiation in mice. It may be concluded that TP (50% EGCG) possess good irradiation-protective and antioxidant effect.
