Sequence analysis of rDNA-ITS of Bai Shouwu from different species.
- Author:
Ning ZHANG
1
;
Bin YAN
;
Xinghang XU
;
Lingchuan XU
Author Information
- Publication Type:Journal Article
- MeSH: Cynanchum; classification; genetics; DNA, Plant; genetics; DNA, Ribosomal Spacer; genetics; Molecular Sequence Data
- From: China Journal of Chinese Materia Medica 2010;35(12):1537-1540
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the genetic diversity of rDNA ITS sequences in different species of Bai Shouwu, utilize the molecular diversity of ITS sequences to authenticate the different species of Bai Shouwu.
METHODFirstly, total DNA was extracted from the different species of Bai Shouwu. Secondly, the ITS sequence was amplified by PCR with universal primer of ITS and sequenced after cloning and purification.
RESULTFrom four species the complete sequence of ITS and 5.8 S rDNA, the partial sequences of 18S rDNA and 26S rDNA were obtained. The rDNA ITS sequences of Cynanchum bungei (sign in No. GU198970 and No. GU479037) were obtained. Ten variable sites among the sequences were found.
CONCLUSIONITS sequence could be used to authenticate the species. The method could be used to identify germplasm resources and authenticate.
