HPLC fingerprint spectrum of honey-fried Radix Glycyrrhizae.
- Author:
Qian ZHOU
1
;
Jia LV
;
Guihai LI
;
Dianhua SHI
;
Yanpeng DAI
;
Lili SUN
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Food Handling; standards; Glycyrrhiza; chemistry; classification; Honey; analysis; Plant Extracts; analysis; Quality Control
- From: China Journal of Chinese Materia Medica 2010;35(12):1547-1550
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the HPLC fingerprint of the pieces of honey-fried Radix Glycyrrhizae.
METHODUsing the reverse-performance liquid chromatography, method was performed on a Hyperclone ODS C18 column (4.6 mm x 250 mm, 5 microm) and acetonitrile-0.1% phosphoric acid was selected as mobile phase gradient elution were adopted.
RESULTEstablished HPLC fingerprint of Radix et Rhizoma glycyrrhizae pieces were established, and the results of methodological study met the technical requirements for fingerprinting.
CONCLUSIONThe HPLC method is stable, accurate, and reliable to provide a scientific basis of quality control standard for the honey-fried Radix et Rhizoma glycyrrhizae.