Effects of ingredients from Chinese herbs with nature of cold or hot on expression of TRPV1 and TRPM8.
- Author:
Feng SUI
1
;
Na YANG
;
Changbin ZHANG
;
Xinliang DU
;
Lanfang LI
;
Xiaogang WENG
;
Shuying GUO
;
Hairu HUO
;
Tingliang JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Drugs, Chinese Herbal; administration & dosage; analysis; Gene Expression; drug effects; Male; Rats; Rats, Sprague-Dawley; TRPM Cation Channels; genetics; metabolism; TRPV Cation Channels; genetics; metabolism
- From: China Journal of Chinese Materia Medica 2010;35(12):1594-1598
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of the ingredients from Chinese herbs with the nature of cold or hot on the expression of TRPV1 and TRPM8.
METHODThe effects of ingredients from herbs on primary culture DRG neurons are observed in vitro. The expression quantity of gene is detected by the method of real time PCR. the 2 (-deltadeltaCT) method is applied to analyze the data.
RESULTIngredients from herbs with the nature of cold up-regulate the expression level of TRPV1 and down-regulate that of TRPM8, especially under the temperature condition of 39 degrees C; while ingredients from herbs with the nature of hot up-regulate the expression level of TRPM8 and down-regulated that of TRPV1, which is more significant under the temperature condition of 19 degrees C.
CONCLUSIONThe regulatory changes of TRPV1 and TRPM8 mRNA expression induced by the chemical ingredients might be related to the cold and hot natures of the herbs from which the ingredients are extracted. And this could be one of the therapeutic mechanisms for the treatment of Chinese herbal medicines to cold- and heat-related diseases.
