Expression changes of age-related genes in different aging stages of Caenorhabiditis elegans and the regulating effects of Chuanxiong extract.
- Author:
Xiaoyan WANG
1
;
Xiangming WANG
;
Danqiao WANG
;
Lianda LI
;
Xiaohong NIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Caenorhabditis elegans; genetics; growth & development; metabolism; Caenorhabditis elegans Proteins; genetics; metabolism; Drugs, Chinese Herbal; pharmacology; Gene Expression Regulation, Developmental; drug effects; Longevity; drug effects
- From: China Journal of Chinese Materia Medica 2010;35(12):1599-1602
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the expression changes of age-related genes in different stages of aging and the regulating effects of Chuanxiong extract on it.
METHODAccording to the different stages of aging, the experiments were tested at two time points of 2 d and 6 d. Using realtime RT-PCR (qRT-PCR) to test the expression change of aging-related genes among the groups.
RESULTCompared with the 2 d control group, the expression of age-1, daf-2, let-363 were up-regulated in the 6 d control group (P < 0.05) while the expression of ins-18, let-60, sir-2.1, sod-3 were down-regulated (P < 0.05). Compared with the 2 d administration group, the expression of age-1, daf-2, let-363 were significantly up-regulated (P < 0.01) in the 6 d administration group after treated with CXE while the expression of ins-18, let-60, sir-2.1, sod-3 were significantly down-regulated (P < 0.01).
CONCLUSIONIn the progress of aging, the expression of age-1, daf-2, let-363 increased, functioning as aging-promoting genes; while the expression of ins-18, let-60, sir-2.1, sod-3 decreased, functioning as longevity genes; CXE extended the lifespan through inhibiting the expression of these aging-promoting genes and increasing the expression of longevity genes, which would be the molecular mechaniSm of anti-aging of traditional Chinese medicine that can promote Qi and activate blood.
