Molecular cloning, characterization and expression analysis of woodchuck retinoic acid-inducible gene I.
10.1007/s11596-016-1588-5
- Author:
Qi YAN
1
;
Qin LIU
1
;
Meng-Meng LI
1
;
Fang-Hui LI
1
;
Bin ZHU
1
;
Jun-Zhong WANG
1
;
Yin-Ping LU
1
;
Jia LIU
1
;
Jun WU
1
;
Xin ZHENG
1
;
Meng-Ji LU
2
;
Bao-Ju WANG
3
;
Dong-Liang YANG
4
Author Information
1. Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
2. Institute of Virology, University of Duisburg-Essen, Essen, 45149, Germany.
3. Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. bjwang-73@163.com.
4. Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. dlyang55@hotmail.com.
- Publication Type:Journal Article
- Keywords:
retinoic acid-inducible gene I;
woodchuck;
woodchuck hepatitis virus
- MeSH:
Animals;
Cell Line, Tumor;
Cloning, Molecular;
DEAD Box Protein 58;
antagonists & inhibitors;
genetics;
immunology;
Fibroblasts;
immunology;
pathology;
Gene Expression;
Hepatitis B;
genetics;
immunology;
pathology;
veterinary;
Hepatitis B Virus, Woodchuck;
Immunity, Innate;
Interferon-beta;
genetics;
immunology;
Isoelectric Point;
Kidney;
immunology;
pathology;
virology;
Liver;
immunology;
pathology;
virology;
Marmota;
genetics;
immunology;
virology;
Open Reading Frames;
Protein Domains;
RNA, Double-Stranded;
RNA, Small Interfering;
genetics;
metabolism;
Rodent Diseases;
genetics;
immunology;
pathology;
virology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2016;36(3):335-343
- CountryChina
- Language:English
-
Abstract:
Cytosolic retinoic acid-inducible gene I (RIG-I) is an important innate immune RNA sensor and can induce antiviral cytokines, e.g., interferon-β (IFN-β). Innate immune response to hepatitis B virus (HBV) plays a pivotal role in viral clearance and persistence. However, knowledge of the role that RIG-I plays in HBV infection is limited. The woodchuck is a valuable model for studying HBV infection. To characterize the molecular basis of woodchuck RIG-I (wRIG-I), we analyzed the complete coding sequences (CDSs) of wRIG-I, containing 2778 base pairs that encode 925 amino acids. The deduced wRIG-I protein was 106.847 kD with a theoretical isoelectric point (pI) of 6.07, and contained three important functional structures [caspase activation and recruitment domains (CARDs), DExD/H-box helicases, and a repressor domain (RD)]. In woodchuck fibroblastoma cell line (WH12/6), wRIG-I-targeted small interfering RNA (siRNA) down-regulated RIG-I and its downstrean effector-IFN-β transcripts under RIG-I' ligand, 5'-ppp double stranded RNA (dsRNA) stimulation. We also measured mRNA levels of wRIG-I in different tissues from healthy woodchucks and in the livers from woodchuck hepatitis virus (WHV)-infected woodchucks. The basal expression levels of wRIG-I were abundant in the kidney and liver. Importantly, wRIG-I was significantly up-regulated in acutely infected woodchuck livers, suggesting that RIG-I might be involved in WHV infection. These results may characterize RIG-I in the woodchuck model, providing a strong basis for further study on RIG-I-mediated innate immunity in HBV infection.
