Downregulation of inwardly rectifying potassium channel 5.1 expression in C57BL/6J cochlear lateral wall.
10.1007/s11596-016-1600-0
- Author:
Chun-Chen PAN
1
;
Han-Qi CHU
1
;
Yan-Bing LAI
1
;
Yan-Bo SUN
1
;
Zhi-Hui DU
1
;
Yun LIU
1
;
Jin CHEN
1
;
Ting TONG
1
;
Qing-Guo CHEN
1
;
Liang-Qiang ZHOU
1
;
Dan BING
1
;
Yan-Ling TAO
2
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
2. Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. 18907183041@163.com.
- Publication Type:Journal Article
- Keywords:
C57BL/6J;
Kir5.1;
cochlea;
presbycusis
- MeSH:
Aging;
genetics;
metabolism;
Animals;
Cations, Monovalent;
Fluorescent Antibody Technique;
Gene Expression Regulation;
Ion Transport;
Mice;
Mice, Inbred C57BL;
Microtomy;
Potassium;
metabolism;
Potassium Channels, Inwardly Rectifying;
genetics;
metabolism;
Presbycusis;
genetics;
metabolism;
physiopathology;
RNA, Messenger;
genetics;
metabolism;
Spiral Ligament of Cochlea;
metabolism;
physiopathology;
ultrastructure
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2016;36(3):406-409
- CountryChina
- Language:English
-
Abstract:
Age-related hearing loss (AHL) is one of the most common sensory disorders among elderly persons. The inwardly rectifying potassium channel 5.1 (Kir5.1) plays a vital role in regulating cochlear K(+) circulation which is necessary for normal hearing. The distribution of Kir5.1 in C57BL/6J mice cochleae, and the relationship between the expression of Kir5.1 and the etiology of AHL were investigated. Forty C57BL/6J mice were randomly divided into four groups at 4, 12, 24 and 52 weeks of age respectively. The location of Kir5.1 was detected by immunofluorescence technique. The mRNA and protein expression of Kir5.1 was evaluated in mice cochleae using real-time polymerase-chain reactions (RT-PCR) and Western blotting respectively. Kir5.1 was detected in the type II and IV fibrocytes of the spiral ligament in the cochlear lateral wall of C57BL/6J mice. The expression levels of Kir5.1 mRNA and protein in the cochleae of aging C57BL/6J mice were down-regulated. It was suggested that the age-related decreased expression of Kir5.1 in the lateral wall of C57BL/6J mice was associated with hearing loss. Our results indicated that Kir5.1 may play an important role in the pathogenesis of AHL.
