Stereotactic injection of shrna GSK-3β-AAV promotes axonal regeneration after spinal cord injury.
10.1007/s11596-016-1623-6
- Author:
Yu-Chao ZUO
1
;
Nan-Xiang XIONG
2
;
Hong-Yang ZHAO
3
Author Information
1. Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
2. Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. mozhuoxiong@163.com.
3. Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. hyzhao750@sina.com.
- Publication Type:Journal Article
- Keywords:
myelin proteins;
neurites;
protein-serine-threonine kinases;
wound healing
- MeSH:
Animals;
Axons;
drug effects;
metabolism;
Dependovirus;
genetics;
Glycogen Synthase Kinase 3 beta;
genetics;
metabolism;
Humans;
Nerve Regeneration;
genetics;
RNA, Small Interfering;
administration & dosage;
genetics;
Rats;
Sensorimotor Cortex;
drug effects;
pathology;
Spinal Cord Injuries;
genetics;
pathology;
therapy;
Syringomyelia;
genetics;
pathology;
therapy
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2016;36(4):548-553
- CountryChina
- Language:English
-
Abstract:
Evidence suggested that glycogen synthase kinase-3β (GSK-3β) is involved in Nogo-66 inhibiting axonal regeneration in vitro, but its effect in vivo was poorly understood. We showed that stereotactic injection of shRNA GSK-3β-adeno associated virus (GSK-3β-AAV) diminished syringomyelia and promoted axonal regeneration after spinal cord injury (SCI), using stereotactic injection of shRNA GSK-3β-AAV (tested with Western blotting and RT-PCR) into the sensorimotor cortex of rats with SCI and by the detection of biotin dextran amine (BDA)-labeled axonal regeneration. We also determined the right position to inject into the sensorimotor cortex. Our findings consolidate the hypothesis that downregulation of GSK-3β promotes axonal regeneration after SCI.
