Effect and mechanism of Bushen Huoxue recipe on ovarian reserve in mice with premature ovarian failure.
10.1007/s11596-016-1627-2
- Author:
Kun-Kun SONG
1
;
Wen-Wen MA
1
;
Cong HUANG
1
;
Jia-Hui DING
1
;
Dan-Dan CUI
1
;
Xiu-Juan TAN
1
;
Jing XIAO
1
;
Ming-Min ZHANG
2
Author Information
1. Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
2. Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. mmzhangeins@163.com.
- Publication Type:Journal Article
- Keywords:
Bushen Huoxue;
Mouse Vasa Homologue;
neo-oogenesis;
ovarian reserve;
premature ovarian failure
- MeSH:
Animals;
Bone Marrow;
drug effects;
metabolism;
Cyclophosphamide;
toxicity;
Disease Models, Animal;
Drugs, Chinese Herbal;
administration & dosage;
Estrous Cycle;
drug effects;
Female;
Gene Expression Regulation;
drug effects;
Humans;
Mice;
Ovarian Follicle;
drug effects;
growth & development;
Ovarian Reserve;
drug effects;
Primary Ovarian Insufficiency;
chemically induced;
drug therapy;
pathology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2016;36(4):571-575
- CountryChina
- Language:English
-
Abstract:
The aim of the present study was to explore the effect and mechanism of Bushen Huoxue recipe (BHR) on ovarian reserve in mice with premature ovarian failure (POF). Mice were divided into 3 groups: normal group, model group and BHR group. Intraperitoneal injection of cyclophosphamide was performed to create the POF model. Primordial follicular (PDF) number, ovarian wet weight, ovarian index, and estrous cycle were analyzed to evaluate the effect of BHR on POF. Meanwhile, the mRNA and protein level of Mouse Vasa Homologue (MVH) in the bone marrow, peripheral blood and ovary were detected, to explore the underlying mechanism of the treatment efficacy of BHR on ovarian reserve. By the time of BHR treatment for 28 days, BHR increased the PDF number and shortened the estrous cycle of POF mice. BHR also decreased the mRNA level of MVH in the bone marrow, and increased mRNA and protein level of MVH in the ovary of POF mice. Our results demonstrated a treatment efficacy of BHR on POF mice, and revealed that BHR might repair the dysfunction of germline stem cells in the bone marrow, and thus to improve the ovarian reserve and enhance the ovarian function of POF mice through neo-oogenesis.
