Inhibition of calpain on oxygen glucose deprivation-induced RGC-5 necroptosis.
- Author:
Shuang CHEN
1
;
Jie YAN
2
;
Hai-Xiao DENG
3
;
Ling-Ling LONG
2
;
Yong-Jun HU
4
;
Mi WANG
1
;
Lei SHANG
5
;
Dan CHEN
1
;
Ju-Fang HUANG
1
;
Kun XIONG
6
Author Information
1. Department of Anatomy and Neurobiology, Central South University, Changsha, 410013, China.
2. Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, 410013, China.
3. Five-year Medicine Program, Grade 2013, Xiangya School of Medicine, Central South University, Changsha, 410013, China.
4. Department of Cardiology, People's Hospital of Hunan Province, Changsha, 410008, China.
5. Jiangxi Research Institute of Ophthalmology and Visual Sciences, Affiliated Eye Hospital of Nanchang University, Nanchang, 330006, China.
6. Department of Anatomy and Neurobiology, Central South University, Changsha, 410013, China. xiongkun2001@163.com.
- Publication Type:Journal Article
- Keywords:
N-acetyl-leucyl-leucyl-norleucinal;
calpain;
necroptosis;
oxygen glucose deprivation;
retinal ganglion cell-5;
truncated apoptosis inducing factor
- MeSH:
Animals;
Apoptosis Inducing Factor;
biosynthesis;
genetics;
Calpain;
biosynthesis;
genetics;
Gene Expression Regulation;
drug effects;
Glucose;
metabolism;
Humans;
Leupeptins;
administration & dosage;
Mice;
Oxygen;
metabolism;
Retinal Ganglion Cells;
metabolism;
pathology;
Retinal Necrosis Syndrome, Acute;
genetics;
pathology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2016;36(5):639-645
- CountryChina
- Language:English
-
Abstract:
The purpose of this study was to investigate the effect of inhibition of calpain on retinal ganglion cell-5 (RGC-5) necroptosis following oxygen glucose deprivation (OGD). RGC-5 cells were cultured in Dulbecco's-modified essential medium and necroptosis was induced by 8-h OGD. PI staining and flow cytometry were performed to detect RGC-5 necrosis. The calpain expression was detected by Western blotting and immunofluorescence staining. The calpain activity was tested by activity detection kit. Flow cytometry was used to detect the effect of calpain on RGC-5 necroptosis following OGD with or without N-acetyl-leucyl-leucyl-norleucinal (ALLN) pre-treatment. Western blot was used to detect the protein level of truncated apoptosis inducing factor (tAIF) in RGC-5 cells following OGD. The results showed that there was an up-regulation of the calpain expression and activity following OGD. Upon adding ALLN, the calpain activity was inhibited and tAIF was reduced following OGD along with the decreased number of RGC-5 necroptosis. In conclusion, calpain was involved in OGD-induced RGC-5 necroptosis with the increased expression of its downstream molecule tAIF.
