Lecithin-cholesterol acyltransferase gene 608C/T polymorphism associated with atherosclerotic cerebral infarction.
- Author:
Xiao-yan ZHU
1
;
Hong-wei XU
;
Rong-yao HOU
;
Heng-fang LIU
;
Bo XIAO
;
Xiao-su YANG
;
Qi-dong YANG
;
Bei-sha TANG
Author Information
- Publication Type:Journal Article
- MeSH: Aged; Alleles; Cerebral Infarction; etiology; genetics; Female; Gene Frequency; Genotype; Humans; Intracranial Arteriosclerosis; complications; Male; Middle Aged; Phosphatidylcholine-Sterol O-Acyltransferase; genetics; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; Polymorphism, Single-Stranded Conformational; genetics
- From: Chinese Journal of Medical Genetics 2006;23(4):419-422
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the distribution of lecithin-cholesterol acyltransferase gene (LCAT) 608C/T polymorphism in Chinese Han population and the relationship of the polymorphism association with the occurrence of atherosclerotic cerebral infarction.
METHODSThe lecithin:cholesterol acyltransferase gene 608C/T polymorphism is identified by polymerase chain reaction (PCR), single-strand conformation polymorphism (SSCP)and restriction fragment length polymorphism (RFLP) in 150 patients with ACI and 122 healthy controls matching age and sex.
RESULTSThe distribution of LCAT 608C/T gene polymorphism was in accordance with Hardy-Weinberg equilibrium. The CT genotype frequency (14.0%) and T allele frequency (7.0%) in ACI group were significantly higher than those in control group (P<0.05). The concentration of high density lipoprotein cholesterol (HDL-C) in 608CC subgroups were significantly higher than those in 608CT subgroups both in ACI group and in control group (P<0.05).
CONCLUSIONThe LCAT 608C/T polymorphism is possibly a predisposing factor in ACI happening of Chinese Han population. T allele frequency is possibly concerned with the metabolism of HDL-C.
