The genetic polymorphism of HLA-DQA1 and HLA-DQB1 genes of Chinese Han population in Jiangsu area is studied by PCR-sequence-based typing.
- Author:
Xin HONG
1
;
Wei-liang DING
;
Yong-fei TAN
;
Guan-ling WU
;
Rong-bin YU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Alleles; Asian Continental Ancestry Group; genetics; China; Female; Gene Frequency; Genotype; HLA-DQ Antigens; genetics; HLA-DQ alpha-Chains; HLA-DQ beta-Chains; Haplotypes; Humans; Male; Middle Aged; Polymerase Chain Reaction; methods; Polymorphism, Genetic; Young Adult
- From: Chinese Journal of Medical Genetics 2006;23(4):463-465
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the polymorphism of HLA-DQA1 and DQB1 genes of Han population in Jiangsu of China.
METHODSThe alleles and haplotypes frequencies of HLA-DQA1 and DQB1 genes in 100 unrelated healthy individuals were analyzed by using polymerase chain reaction-sequence-based typing (PCR-SBT).
RESULTSAmong the 7 DQA1 alleles detected, the most common allele was DQA1*0301/02/03 with a frequency of 29.5%, which was followed by DQA1*0501, DQA1*0102 and DQA1*0201 with frequencies of 18.5%, 17.0% and 12.5%, respectively. Of the 13 DQB1 alleles detected, DQB1*0201/02 allele (21.5%) was the most frequent allele, followed by DQB1*0301/09 (14.5%), DQB1*0303 (13.5%) and DQB1*0603 (11.5%). The most common DQA1 vs DQB1 haplotype was DQA1*0301/02/03 vs DQB1*0303 with a frequency of 12.5%, which was followed by the DQA1*0201-DQB1*0201/02 (10.5%),DQA1*0501-DQB1*0201/02 (9.5%) and DQA1*0501-DQB1*0301/09 (7.0%).
CONCLUSIONThe distribution of HLA-DQ alleles and haplotypes in Jiangsu Han population shares some genetic characteristics with other population in northern of China, but has its own characteristics. The data will provide useful information for anthropology, organ transplantation and disease association studies.
