Study on the association of polymorphisms in homocysteine metabolism related enzymes with deep venous thrombosis.
- Author:
Hai-dong YU
1
;
Hong ZHENG
;
Hua QI
;
Jian-hua LIAN
;
Ying HE
;
Zi-ming DONG
Author Information
- Publication Type:Journal Article
- MeSH: 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase; genetics; Adult; Alleles; Cystathionine beta-Synthase; genetics; Female; Gene Frequency; Genetic Predisposition to Disease; Genotype; Haplotypes; Humans; Male; Methylenetetrahydrofolate Reductase (NADPH2); genetics; Middle Aged; Point Mutation; Polymorphism, Genetic; Venous Thrombosis; enzymology; genetics
- From: Chinese Journal of Medical Genetics 2006;23(6):635-639
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the significance of gene mutation of methylenetetrahydrofolate reductase (MTHFR) C677T, methionine synthase (MS) 2756 AG and cystathionine beta-synthase (CBS) 844ins68 in the development of deep venous thrombosis.
METHODSOne hundred and three cases of deep venous thrombosis (DVT group) and 250 healthy subjects (control group) were recruited in the study. The polymorphisms of MTHFR C677T, MS A2756G and CBS 844ins68 were detected by PCR-restriction fragment length polymorphism(PCR-RFLP).
RESULTSThe prevalences of TT genotypes of MTHFR (C677T) between DVT group and normal control group had significant difference (27.2% vs 17.2%, P< 0.05), the prevalence of AG genotypes of MS A2756G in the DVT group was less than that in the control group (9.7% vs 19.2%, P< 0.05). The prevalence of 677T-2756A haplotype in the DVT group was higher than that in the control group (P< 0.05), the prevalence of 677C-2756A haplotype in the DVT group was less than that in the control group (P< 0.05). There were no significant differences in the prevalences of CBS 844ins68 mutation.
CONCLUSIONThe homozygote of MTHFR C677T (TT) may be a risk factor of DVT. MS A2756 G(AG) genotypes may reduce the development of DVT. The 677T-2756A haplotype may be a risk factor of DVT. The 677C-2756A haplotype may be a protective factor of DVT. The prevalence of gene mutation of CBS 844ins68 might vary with different ethnic group or geographic regions.
