Prevalence and clinical characteristics of the A to G variant at position 12026 of the mitochondrial ND4 gene in familial diabetes mellitus in Chinese population.
- Author:
Sui-jun WANG
1
;
Song-hua WU
;
Tai-shan ZHENG
;
Zhen YANG
;
Hui-juan LU
Author Information
- Publication Type:Journal Article
- MeSH: Asian Continental Ancestry Group; genetics; Blood Glucose; metabolism; Body Mass Index; China; DNA, Mitochondrial; chemistry; genetics; Diabetes Mellitus; blood; ethnology; genetics; Family Health; Female; Gene Frequency; Humans; Male; Middle Aged; NADH Dehydrogenase; genetics; Point Mutation; Polymorphism, Genetic; Sequence Analysis, DNA
- From: Chinese Journal of Medical Genetics 2006;23(6):652-654
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assess the prevalence of the A to G variant at nucleotide 12026 (mt12026) of the mitochondrial NADH-dehydrogenase subunit 4 (ND4) gene in familial diabetes mellitus in Chinese population.
METHODSThe authors screened 770 randomly selected, unrelated probands of diabetic pedigrees, and 309 controls with normal glucose tolerance for the variant by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique and PCR-direct-sequencing.
RESULTSThe mt12026 A --> G variant was detected in 28 diabetic patients (3.63%) and 9 controls (2.91%). The frequency of the variant mt12026 A --> G was not statistically different between diabetic patients and controls. Moreover, clinical characteristics such as age, body mass index (BMI), and insulin resistant index were not different between diabetic patients with and without the mt12026 mutation.
CONCLUSIONThe mt12026 A --> G variant is a mitochondrial gene polymorphism in Chinese population, and it is unlikely that the mutation is in itself the cause of diabetes.
