The relationship between resting heart rate and single nucleotide polymorphism at 3 sites of nitric oxide synthase gene in the Chinese Han nationality population.
- Author:
Hou-xun MA
1
;
Yong-hong NIU
;
Zhang-yong LI
;
Zheng-xiang XIE
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Asian Continental Ancestry Group; genetics; China; Female; Gene Frequency; Genotype; Heart Rate; Humans; Male; Middle Aged; Nitric Oxide Synthase; genetics; Nitric Oxide Synthase Type II; genetics; Nitric Oxide Synthase Type III; genetics; Polymorphism, Single Nucleotide
- From: Chinese Journal of Medical Genetics 2006;23(6):670-672
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the relationship between resting heart rate and single nucleotide polymorphisms (SNP) at 3 sites of nitric oxide synthase (NOS) gene including NOS3 -922A/G, NOS3 894G/T and NOS2 -1173C/T SNPs.
METHODSGenomic DNA was gained from 211 Chinese Han nationality population. The SNPs of NOS3 -922A/G, NOS3 894G/T and NOS2 -1173C/T were genotyped by allele-specific primer-polymerase chain reaction (ASP-PCR) technique.
RESULTSThe distribution frequencies of GG, GT and TT genotypes of NOS3 894G/T and AA, AG and GG genotypes of NOS3 -922A/G and CC, CT and TT genotypes of NOS2 -1173C/T were consistent with Hardy-Weinberg equilibrium (P> 0.05). The resting heart rate of Chinese Han nationality population with AA genotypes was higher than that with GG genotype of NOS3 -922A/G (P< 0.01). The resting heart rate of the sub-population with GG genotype was higher than that with TT genotype of NOS3 894G/T (P< 0.05). There were no difference among the resting heart rates of the sub-populations with the allele genotypes of NOS2 -1173C/T.
CONCLUSIONThe resting heart rate of Chinese Han nationality population with mutated genotype GG of NOS3 -922A/G and with mutated genotype TT of NOS3 894G/T were lower than those with wild genotype of NOS3 -922A/G and NOS3 894G/T. The finding suggests that resting heart rate is associated with SNP of NOS3 -922A/G and NOS3 894G/T.
