Polymorphic analysis of two Y chromosome short tandem repeat loci in Chinese Chengdu Han population.
- Author:
Jing-dong LIN
1
;
Ai-ming HUANG
;
Jian-wen HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Asian Continental Ancestry Group; genetics; China; Chromosomes, Human, Y; genetics; Female; Forensic Genetics; methods; Gene Frequency; Haplotypes; Humans; Male; Paternity; Polymerase Chain Reaction; Polymorphism, Genetic; Tandem Repeat Sequences
- From: Chinese Journal of Medical Genetics 2006;23(6):683-684
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo reveal the distribution of genetic polymorphism of two novel Y chromosome short tandem repeat (Y-STR) loci (DYS508, DYS516) in Chinese Chengdu Han population.
METHODSTwo Y-STR loci were amplified with PCR. The products of PCR were analyzed with polyacrylamide gel electrophoresis. Each allele of these 2 Y-STR loci was sequenced and the allele ladders were constructed. Alleles of these Y-STR loci were nominated according to recommendations of the International Society of Forensic Genetics (ISFG).
RESULTSSix and 6 alleles were observed in loci DYS508 and DYS516 respectively. The gene diversity of DYS508 and DYS516 was 0.7242 and 0.7931 respectively. The diversity of haplotype for these Y-STR loci was 0.9397. The value of discrimination power and the exclusion chance of paternity for these Y-STR loci were same as the value of haplotype diversity.
CONCLUSIONBoth Y-STR loci are high genetic polymorphic. They are beneficial to the test of male-female mixtures and paternity identification in forensic science.
