Influence of maternal age on meiotic spindle and chromosome configuration of oocytes.
- Author:
Wen CHEN
1
;
Juan HU
;
Qun LIU
;
Yu-lan WEI
;
Gui-jin ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Age Factors; Chromosomes, Human; metabolism; Female; Fertilization in Vitro; Humans; Immunohistochemistry; Microscopy, Confocal; Oocytes; metabolism; Sperm Injections, Intracytoplasmic; Spindle Apparatus; metabolism
- From: Chinese Journal of Medical Genetics 2007;24(1):6-9
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of maternal age on meiotic spindle and chromosome configuration of oocytes.
METHODSSpindle and chromosome configuration was examined in day 1 unfertilized human oocytes after in vitro fertilization (IVF) and intracytoplasmic sperm injection(ICSI) by immunocytochemistry and visualized by laser confocal microscopy.
RESULTSStatistically significant differences were observed on normal spindle and chromosome configurations of oocytes between 25-29 maternal age group (33% and 31%, respectively), and 30-34 age group (P< 0.05) as well as 35-40 age group(0%, P<0.01). The incidence of abnormal spindle and chromosome configurations of oocytes from 30-34 and 35-40 maternal age groups was much higher than that of oocytes from 25-29 age group (P<0.01, P<0.05).
CONCLUSIONIncidence of abnormal spindle and chromosome configuration of oocytes is related to maternal age. It could be an important reason of age related oocyte aneuploidy.
