Polymorphism of killer cell immunoglobulin-like receptor 2DL3 gene in Zhejiang Han population.

Author: Fa-ming ZHU ¹ ; Kan JIANG ; Qin-feng LV ; Wei ZHANG ; Ji HE ; Xian-guo XU ; Li-xing YAN
Author Information

1. Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang, 310006 PR China.
Publication Type:Journal Article
MeSH: Alleles; Base Sequence; China; Gene Frequency; Genotype; Humans; Polymerase Chain Reaction; Polymorphism, Genetic; genetics; Receptors, KIR2DL3; genetics; Sequence Analysis, DNA
From: Chinese Journal of Medical Genetics 2007;24(1):88-90
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo analyze the allelic polymorphism of killer cell immunoglobulin-like receptor 2DL3 (KIR2DL3) gene in Zhejiang Han population.
METHODSAllelic of KIR2DL3 was detected by PCR sequence based typing (PCR-SBT) method. Two specific fragments of KIR2DL3 were amplified using different primers and sequenced for exons 4,5,7,8,9 of KIR2DL3. All the samples would be assigned to KIR2DL3 allele according to the polymorphism site patterns.
RESULTSTwo KIR2DL3 alleles were observed, with KIR2DL3*001 having higher frequency, 0.77.
CONCLUSIONThe PCR-SBT method is reliable and there are distinctive frequencies of KIR2DL3 alleles in Zhejiang Han population.