Quantification of α-methylene-λ-butyrolactone extracted from different parts ofAlstroemeria wilhelmina and evaluation of it's antigenicity using the guinea-pig maximization test.
- Author:
Koichi HARADA
1
;
Shoko OHMORI
;
Chang-Nian WEI
;
Yoshiki ARIMATSU
;
Atsushi UEDA
Author Information
- Publication Type:Journal Article
- Keywords: Alstroemeria wilhelmina; contact dermatitis; guinea-pig maximization test (GMT); high-performance liquid chromatography (HPLC); α-methylene-λ-butyrolactone (α-MBL)
- From:Environmental Health and Preventive Medicine 2002;6(4):229-234
- CountryJapan
- Language:English
- Abstract: To detect the type of contact dermatitis caused due to the handling ofAlstroemeria wilhelmina, 1% α-methylene-λ-butyrolactone (α-MBL) dissolved in physiological alien and a five-fold diluted saline solution of original extracts of flowers, leaves and stems of the flower were applied to guinea-pigs for extracts were applied to the animals as the challenge treatment in compliance with the guinea-pig maximization test (GMT). As a consequence, not only primary irritant dermatitis was observed, but also delayed type allergic contact dermatitis due toAlstroemeria wilhelmina was observed. α-MBL determined in the extracts using high-performance liquid chromatography (HPLC) was found to be the biochemical material cause of the contact dermatitis. the flower region contained α-MBL in the highest concentrations compared with those of the leaves and stems. Therefore, the quantification of α-MBL in the extracts was concluded as being a useful evaluating method for contact dermatitis due to the handling ofAlstroemeria.
