Study on HPLC specific chromatograms of Lu Dangshen.
- Author:
Lin-jing GUAN
;
Yun-lan LIAN
;
Jian-kuan LI
;
Xian ZHEN
;
Xian-hai SHI
;
Jian-ping GAO
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Codonopsis;
chemistry;
Drugs, Chinese Herbal;
analysis;
Glucosides;
analysis;
Phenylpropionates;
analysis;
Plant Roots;
chemistry;
Quality Control
- From:
China Journal of Chinese Materia Medica
2015;40(14):2854-2861
- CountryChina
- Language:Chinese
-
Abstract:
In this paper, the RP-HPLC specific chromatography was adopted, with DIKMA-C18 (4.6 mm x 250 mm, 5 µm) as the chromatographic column, with a gradient elution compose of acetonitrile and 0.1% phosphoric acid at flow rate of 0.8 mL · min(-1), the detection wavelength was 220 nm. The difference of the HPLC specific chromatograms between the Lu Dangshen and other different base sources and different producing area of Codonopsis Radix was compared, involved in the similarities and differences of the number and the relative peak area of characteristic peaks in the HPLC specific chromatograms. The HPLC specific chromatograms of Lu Dangshen was established and the relative retention times of seven peaks was determined, and the peaks of codonopyrrolidium B, syringin, lobetyolin, tangshenoside I and atractylenoide III were identified; The HPLC specific chromatograms of Lu Dangshen provided a method for scientific evaluation and effective control the quality of Lu Dangshen from Shanxi famous-region.
