Rat intestine absorption kinetics study on cucurbitacin B-sodium deoxycholate/phospholipid mixed nanomicelles with in vitro everted gut sacs model.
- Author:
Ling CHENG
;
Bao-de SHEN
;
Juan-juan LI
;
Ling QIU
;
Gang SHEN
;
Li-hong ZHANG
;
Jin HAN
;
Hai-long YUAN
- Publication Type:Journal Article
- MeSH:
Animals;
Deoxycholic Acid;
administration & dosage;
Female;
Intestinal Absorption;
Kinetics;
Male;
Micelles;
Nanoparticles;
Phospholipids;
administration & dosage;
Rats;
Rats, Wistar;
Triterpenes;
administration & dosage;
pharmacokinetics
- From:
China Journal of Chinese Materia Medica
2015;40(14):2876-2881
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the absorption kinetics of Cu B-SDC/PLC-MMs in rat different intestinal segments and compared with the absorption of Cu B suspension. The in vitro everted gut sacs model was established to study the absorption characteristics of Cu B-SDC/ PLC-MMs in rat duodenum, jejunum, ileum and colon, and the content of cucurbitacin B was detected by HPLC method, and the effects of concentrations on intestinal absorption were evaluated as well. The results showed that the absorption of Cu B-SDC/PLC-MMs was linearity at different intestine segment and different concentrations (R2 > 0.9), which was consistent with zero order rate process. The Ka of different intestine segments showed a concentration-dependent increasing along with the raised concentration of Cu B-SDC/ PLC-MMs, indicating that it was likely to be a mechanism of passive absorption. The best absorption site of Cu B-SDC/PLC-MMs was ileum, and its absorptions in different intestinal segments were superior to cucurbitacin B suspension. SDC/PLC-MMs could significantly enhance the intestinal absorption of cucurbitacin B, and the study of intestinal absorption kinetics of Cu B-SDC/PLC-MMs had gave a support to its further reasonable solidfication.
