Chemical constituents and cytotoxicity assay research in small polar substances from Vitis thunbergii var. taiwaniana.
- Author:
Chao JIANG
;
Wen-zhu WANG
;
Xiao-jun LIAO
;
De-quan ZENG
;
Ting LING
;
Shi-lan XU
;
Jin-zhang ZENG
;
Hai-feng CHEN
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents, Phytogenic;
pharmacology;
Humans;
MCF-7 Cells;
Plant Extracts;
analysis;
pharmacology;
Vitis;
chemistry
- From:
China Journal of Chinese Materia Medica
2015;40(15):2999-3004
- CountryChina
- Language:Chinese
-
Abstract:
This article studied the chemical constituents from the aerial part of Vitis thunbergii var. taiwaniana. The 60% ethanol extract was eluted with 95% ethanol though HP-20 macroporous adsorption resin column. 12 compounds, including (1) betulinic acid, (2)2, 2, 2'-bis (4-hydroxyphenyl) propane bis (2, 3-epoxypropyl) ether, (3) eriodictyol, (4) trans-ε-viniferin, (5) (+)-cis-ε-viniferin, (6) kobophenol A, (7) ampelopsin A, (8) nepalensinol B, (9) cis-miyabenol C, (10) cis-vitisin B, (11) cis-gnetin H and (12) (+)-hopeaphenol, were separated by using normal phase silica gel, ODS, Sephdadex LH-20 column chromatographies and semi-preparative or preparative HPLC. Compounds 2, 5, 6, 8, 9, 10, 11 were separated from the genus Vitis for the first time and compounds 3, 7, 12 were separated from Vitis thunbergii var. taiwaniana for the first time. At a concentration of 50 μmol · L(-1), compound 6, 7 and 11 showed strong cytotoxicity against MCF-7 cell lines with the inhibition rate of 66.58%, 57.16%, 52.84%, respectively.
