Multi-index integrated evaluation method optimizes water-extraction and ethanol precipitation of Xuanbi'antong formula (XBF).
- Author:
Heng-Wen CHEN
1
;
Cui-Ying ZHANG
1
;
Wei-Guang REN
1
;
Jie WANG
1
;
Ben-Jun WEI
1
Author Information
- Publication Type:Journal Article
- Keywords: Xuanbi′antong (XBF); multi-index evaluation; orthogonal test; water-extraction and ethanol precipitation process
- From: China Journal of Chinese Materia Medica 2016;41(1):70-74
- CountryChina
- Language:Chinese
- Abstract: To determine the process parameters of optimal water-extraction and ethanol precipitation method for Xuanbi'antong (XBF) extract, which is a clinically experience formula for coronary disease. Orthogonal test L9(34) was conducted for the study of XBF water-extraction and ethanol precipitation process. Extractum, salvianolic acid B, rhizoma coptidis alkaloid, paeoniflorin, puerarin, ginsenoside Rb₁, ginsenosides and echinacoside were selected as marker components and multi-index comprehensive weighted score was used to select and verify optimal water-extraction and ethanol precipitation process. The optimal extraction process was as follows： XBF was added with 10 times distilled water, decocted for half an hour for 3 times. The best ethanol-precipitation process was established where the ethanol was added up to 70% and precipitated for 24 hours in 1.12 extract density (20 ℃). The optimized water-extraction and ethanol precipitation method is stable and reliable, and can provide reference for further development and utilization of the formula.
