Establishment of Lymphangioma Model and a Study on the Promoting Effect of Murine Melanoma Cell B16-F1 on the Lymphangiogenesis In Vitro
- Author:
Siyuau CHEN
1
;
Aijun CHEN
;
Chaugzheng HUANG
;
Yue QIAN
;
Zhixiang LIU
;
Yan WU
;
Yating TU
Author Information
1. 华中科技大学同济医学院附属协和医院
- Keywords:
melanoma;
lymphangiogenesis;
lymphatic metastasis
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2007;27(6):733-735
- CountryChina
- Language:Chinese
-
Abstract:
To establish an animal model of benign lymphangiomas of C57BL/6 mouse in vitro and to observe the effect of mouse ascites melanoma cell B16-F1 on the lymphangiogenesis, 16 C57BL/6 mice aged 8 weeks were given two intraperitoneal injections of incomplete Freund's adjuvant at a15-day interval. The induced neoplasms were studied histopathologically and thhe neoplasms speci- mens were immunohistochemically examined for the expressions of VEGF-C (vascular endothelial growth factor-C) and Fit-4 (VEGFR-3, vascular endothelial growth factor receptor-3). The neoplasms were harvested and embedded in fibrin gel for culture in conditioned medium of B16-F1 cells in vitro and observed under inverted microscope. Our results showed that white solid tumor masses devel- oped in peritoneal cavity after the induction. The tumors were confirmed to be lymphangioma by gross and histological examination. The tumor cells expressed both VEGF-C and Flt-4. Lymphatic capillaries coming from lymphangioma specimen grew into the gel and the conditioned medium of B16-F1 cells was found to be able to promote the growth of the vessels. It is concluded that intrap- eritoneal injection of incomplete Freund's adjuvant is a good method for inducing benign lymphan- giomas in mouse and B16-F1 cells can promote lymphangiogenesis.
