Comparative Study on the Effects of Serf-made Liver Preservation Solution on Secretion of ICAM-1 and NO in Rats
- Author:
WANG SHIMING
1
;
DAI PENG
;
XU JUN
;
YANG ZHENG
Author Information
1. 华中科技大学同济医学院
- Keywords:
preservation solution;
liver transplantation;
intercellular adhesion molecule-1;
nitric oxide
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2008;28(1):77-79
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the effect of self-made liver preservation solution on liver preservation by comparing with UW solution and HC-A solution, the self-made liver preservation solution (SM) and perfusion solution were prepared under the aseptic conditions. The isolated non-circulated perfu-sion rat liver model was established. According to the different preservation solutions, the rats were randomly divided into UW group, SM group and HC-A group. The three groups were divided into 6subgroups according to the preservation duration (n=6 in each group). The transferase in liver perfu-sion solution and intercellular adhesion molecule-1 (ICAM-1) and nitric oxide (NO) in liver tissues were determined at 2, 8 and 24 h respectively. The results showed that the levels of alanine aml- notransferase (ALT) and aspartate aminotransferase (AST) had no significant difference between SM group and UW group, but significantly lower than in HC-A group. The levels of ICAM-1 and NO were increased simultaneously in SM group and UW group (P＞0.05), but there was significant dif-ference as compared with HC-A group (P＜0.05). At the same time point, the level of ICAM-1 was higher in SM group than in UW group, but NO was lower. The preservation effect of SM solution is the same as UW solution, but better than HC-A solution.
