beta-catenin/Tcf signaling in squamous differentiation of porcine airway epithelial cells.
10.1007/s11596-008-0201-y
- Author:
Wenshu CHEN
1
;
Renliang WU
;
Xi WANG
Author Information
1. Institute of Pathology, Tongji Hospital, Department of Pathology, Tongji Medical College, Huazhong University of Science and Technology, Tongji, China. cwenshu@gmail.com
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Differentiation;
Cell Nucleus;
metabolism;
Cells, Cultured;
Cornified Envelope Proline-Rich Proteins;
Cytoplasm;
metabolism;
Epithelial Cells;
cytology;
metabolism;
Membrane Proteins;
metabolism;
Mutation;
RNA, Messenger;
metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Signal Transduction;
Swine;
Trachea;
cytology;
metabolism;
Transcription, Genetic;
beta Catenin;
metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2008;28(2):121-124
- CountryChina
- Language:English
-
Abstract:
For a preliminary study of the role of beta-catenin/Tcf signaling in squamous differentiation of airway (tracheobronchial) epithelial cells, a stable mutant of beta-catenin was transfected into primarily cultured porcine airway epithelial cells. Western blotting revealed that exogenous protein was observed in large quantity in cytoplasm and nucleus. When co-transfected with Tcf luciferase reporter plasmids, beta-catenin mutant increased the reporter's transcriptional activities. However, mRNA expression of a squamous differentiation marker, small proline-rich protein (SPRP), was not elevated, as shown by reverse transcription-polymerase chain reaction. These findings suggest that beta-catenin/Tcf signaling may not be directly involved in the squamous differentiation of porcine airway epithelial cells.
