Expression of Twist Gene in Human Hepatocellular Carcinoma Cell Strains of Different Metastatic Potential
- Author:
ZHU QIAN
1
;
XU HUBO
;
XU QIAN
;
YAN WEI
;
TIAN DE'AN
Author Information
1. 华中科技大学同济医学院附属同济医院
- Keywords:
hepatocellular carcinoma;
Twist gene;
metastasis;
fluorescent polymerase chain reaction
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2008;28(2):144-146
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the role of Twist gene in the metastasis of hepatocellular carcinoma (HCC), total RNA was respectively extracted from three HCC cell strains with different metastatic potentials, HepG2, MHCC-97L and MHCC-97H. The first strand eDNA was synthesized by reverse transcription, which was then used as template to perform fluorescent quantitative polymerase chain reaction (FQ-PCR). The quantity of Twist gene expression was normalized by that of the housekeeping gene, GAPDH for each sample. ANOVA was used to estimate the relationship between Twist gene and metastasis potential of HCC. The results showed that the normalized initial eDNA concen- trations of Twist gene in HepG2, MHCC-97L and MHCC-97H were (9.45±0.25)×104, (1.82±0.41)×10-3, (3.06±0.62)×10-3, respectively. FQ-PCR revealed significant differences in the ex- pression level of Twist among HCC cell strains with different metastatic potentials. It was concluded that high expression level of Twist was closely associated with more aggressive behaviors of HCC. Twist provides a novel indicator for HCC metastasis.
