Relationship between the Expression of RASSF1A Protein and Promoter Hypermethylation of RASSF1A Gene in Bladder Tumor
- Author:
HU JIANTING
1
;
LI HONGZHAO
;
SHI TAOPING
;
MA XIN
;
WANG BAOJUN
;
XU HUA
;
AI XING
;
JU ZHENGHUA
;
WANG CHAO
;
ZHANG GUOXI
;
ZHANG XU
Author Information
1. 华中科技大学同济医学院附属同济医院
- Keywords:
bladder transitional cell carcinoma;
DNA methylation;
Western-blotting;
RASSF1A gone
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2008;28(2):182-184
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the relationship between the expression of RASSFIA protein and promoter hypermethylation of RASSFIA gene, RASSFIA protein expression was measured by Western blot- ting in 10 specimens of normal bladder tissues and 23 specimens of bladder transitional cell carci- noma (BTCC). The promoter methylation in BTCC and normal bladder tissues was detected by me- thylation-specific PCR (MSP). The results showed that the expression level of RASSFIA protein was significantly lower in BTCC tissues than that in normal bladder tissues. However, it was not corre- lated with its clinical stages and pathological grades. The frequency of promoter methylation of RASSF1A gene was higher in BTCC tissues than that in normal bladder tissues. In 14 patients with the aberrant promoter methylation, 13 showed loss or low expression of RASSF1A protein. It is con- cluded that RASSFIA gene promoter methylation may contribute to the low level or loss of RASSFIA protein expression, the inactivation of RASSFIA gene and the genesis of BTCC. But, it may bear no correlation with its clinical stages and pathological grades.
