Inhibitory Activity of Nuclear Factor-κB Potentiates Cisplatin-induced Apoptosis in A549 Cells
- Author:
ZHANG JIAN
1
;
XU YONGJIAN
;
XIONG WEINING
;
ZHANG ZHENXIANG
;
DU CHUNLING
;
QIAO LIFEN
;
NI WANG
;
CHEN SHIXIN
Author Information
1. 华中科技大学同济医学院附属同济医院
- Keywords:
nuclear factor-kappaB;
lung neoplasms;
cisplatin;
apoptosis
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2008;28(3):251-256
- CountryChina
- Language:Chinese
-
Abstract:
Whether inhibiting the activity of nuclear factor (NF)-κB potentiates cisplatin-induced apoptosis in non-small cell lung cell line A549 cells was investigated. The recombinant plasmid pcDNA3.1(+)/IκBα expressing IκBα was constructed. The in vitro cultured A549 cells were trans-fected with pcDNA3.1(+)/IκBα alone, or pcDNA3.1(+)/IκBα combined with cisplatin. The mitochondrial membrane potential (△ψm) was determined by rhodamine 123, the activity of caspase-3 was tested by colorimetric assay, and cell apoptosis was detected by flow cytometry with the annexin V/propidium iodide assay. The results showed that the activity of NF-κB in A549 cells was inhibited by transfecting pcDNA3.1(+)/IκBα. Transfection of pcDNA3.1(+)/IκBα alone did not promote apoptosis. Treatment of cisplatin alone had a little effect on cell apoptosis. Transfection of pcDNA3.1(+)/IκBα combined with cisplatin treatment significantly induced apoptosis of A549 cells. It was concluded that inhibiting the activity of NF-κB potentiated cisplatin-induced apoptosis of A549 cells.
