Effect on Survivin Regulation of Transcription Level by p21waf1 Overexpression in HepG2 Hepatocellular Carcinoma Cells
- Author:
XIONG JUAN
1
;
HU LIHUA
;
LI YIRONG
;
DOU LIFANG
;
CAI PENGCHENG
;
TANG ZHAOMING
;
WANG LIN
Author Information
1. 华中科技大学同济医学院附属协和医院
- Keywords:
p21Cip1/Waf1;
survivin;
hepatocellular carcinoma;
gene expression regulation
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2008;28(3):308-313
- CountryChina
- Language:Chinese
-
Abstract:
The effect of cyclin-dependent kinase inhibitors Cip1/Wafl (p21) on regulatory expression of survivin transcription in human hepatocellular carcinoma cell HepG2 was observed and the related mechanisms explored. Doxorubicin (DOX) was used to treat HepG2. Eukaryotic vector pEGFP-C2-p21 was transfected into HepG2 by lipofectamine and positive clones were screened out by G418. The mRNA expression of p21 and survivin was detected by real-time fluorescent quantitative polymerase chain reaction (RQ-PCR). Flow cytometry was used to examine the cell cycle, and reverse transcription polymerase chain reaction (RT-PCR) was used to measure the levels of E2F-1 and p300. The results showed that: (1) After treatment with DOX, the expression of p21 was increased, whereas that of survivin was reduced during 24h of treatment; (2) After transfection of pEGFP-C2-p21 into HepG2, p21 level was significantly enhanced to 2100.11-folds or 980.89-folds in comparison to HepG2 or HepG2-C2 group, and survivin level was markedly down-regulated to 0.54% or 0.59% relative to the control groups; (3) Overexpressed p21 resulted in G1/G0 phase arrest (F=31.59,P<0.01), meanwhile E2F-1 mRNA and p300 mRNA were reduced as compared with those of controls (FE2F-1=125.28,P<0.05;Fp300=46.01,P<0.01). It was suggested that p21 could be a potential mediator of survivin suppression at transcription level in HepG2 cell, which might be through the block at G1/G0 phase and down-regulation of transcription factors E2F-1 and p300.
