Effects of Oxymatrine on the Apoptosis of Human Esophageal Carcinoma Eca109 Cell Line and Its Mechanism
- Author:
JIN YI
1
;
HU JIANLI
;
WANG QIONG
;
LI ZHENYU
;
CHEN YESHAN
Author Information
1. 华中科技大学同济医学院附属协和医院
- Keywords:
Oxymatrine;
esophageal carcinoma;
extracellular signal-regulated kinase;
apoptosis
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2008;28(3):314-316
- CountryChina
- Language:Chinese
-
Abstract:
The effects of Oxymatrine (Oxy) on the proliferation and apoptosis of human esophageal carcinoma Eca109 cell line and the mechanism were investigated. The human esophageal carcinoma Eca109 cells were cultured in vitro. The Oxy-induced apoptosis of Eca109 cells was assayed by using flow cytometry. The expressions of p-ERK1/2, Cyclin D1, p21waf/cip1, Bax and Bcl-2 were detected by Western blot. Flow cytometry revealed that Oxy could induce the apoptosis of Eca109 cells. Western blot showed that Oxy of different concentrations suppressed the expressions of p-ERK1/2, cyclin D1 and Bc1-2, but up-regulated the expression of p21waf/cip1 and Bax, and the ratio of Bax/Bcl-2 was increased It was suggested the Oxy could induce the apoptosis of Eca109-cells, which might be related to the upregulation of p21waf/cip1 and the downregulation of p-ERK1/2 Cyclin D1 and p21waf/cip1. The possible pathway may be related to Bcl-2/Bax.
