Induction of SiHa Cells Cells Apoptosis by Nanometer Realgar Suspension and Its Mechanism
- Author:
LIU RONG
1
;
PU DEMIN
;
LIU YAN
;
CHENG YANXIANG
;
YIN LING
;
LI TIAN
;
ZHAO LIBO
Author Information
1. 华中科技大学同济医学院附属同济医院
- Keywords:
nanometer realgar suspension;
SiHa cells;
Apoptosis;
Human papilloma virus 16;
E6 gene;
E7 gene
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2008;28(3):317-321
- CountryChina
- Language:Chinese
-
Abstract:
The effects of nanometer realgar suspension on proliferation and apoptosis of human uterine cervix cancer cell line SiHa cells and oncogenic genes HPV16E6/E7 were investigated. A "micro-jet efflux" strategy was used for the preparation of nanometer realgar suspension. SiHa cells were treated with nanometer Realgar suspension in various concentrations (6.25,12.5,25 and 50mg/L) for different durations (12,24,48 and 72h). The inhibitive effect of nanometer realgar suspension on growth of SiHa cells was detected by MIT method. Special morphological changes of apoptosis were observed by transmission electron microscopy (TEM) and DNA fragments electrophoresis. The apoptotic rate was quantified by flow cytometry (FCM). The expression of HPV16E6/E7 mRNA and protein was assayed by RT-PCR and Western blot respectively. The results showed after being treated with 25-50mg/L nanometer realgar suspension for 48h, the survival rate of SiHa cells was decreased, and apoptotic rate markedly increased in a time- and concentration-dependent manner. TEM and DNA electrophoresis revealed the special morphological changes of apoptosis. The apoptotic rate of SiHa cells treated with nanometer realgar suspension was significantly higher than in the control group (P<0.01), and G0/G1 phase arrest appeared following treatment with nanometer realgar suspension in 25 and 50mg/L for 48h.RT-PCR and Western blot assay indicated that nanometer realgar suspension reduced the HPV16E6/E7 gene expression. Nanometer realgar suspension could inhibit the proliferation and induce apoptosis of SiHa cells. The mechanism may be related to the down-regulation of the HPV16E6/E7 gene expression.
