Immune Killing Activity of Lymphocytes on Hela Cells Expressing Interleukin-12 In Vitro

Author: WANG HUIYAN ¹ ; CHEN SUHUA
Author Information

1. 武汉理工大学附属医院
Keywords: interleukin-12; Hela cel1; immune killing activity
From: Journal of Huazhong University of Science and Technology (Medical Sciences) 2008;28(3):343-345
CountryChina
Language:Chinese
Abstract: The killing effects of lymphocytes on Hela cells expressing intedeukin-12 (IL-12) in vitro were explored. By using gene transfection technique, full length IL-12 gene was transfected into Hela cells. The expression of IL-12 in Hela cells was detected quantitatively by ELISA; Changes in killing effects of lymphocytes on Hela cells expressing IL-12 were observed by MTT. It was found that Hela cells could express IL-12 between 24h and 72h after transfection. Killing activity of lymphocytes on Hela cells expressing IL-12 was significantly enhanced. It was concluded by cell transfection technique, Hela cells could express IL-12 and were more easily killed by lymphocytes.