Effect of gardenin on gene expression profile in brain of rats with focal cerebral ischemia.
- Author:
Xiao-yan ZHANG
1
;
Zhan-jun ZHANG
;
Zhong WANG
;
Kang YING
;
Ming-xue ZUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain Ischemia; genetics; metabolism; Gene Expression Profiling; Gene Expression Regulation; drug effects; Male; Pyridines; pharmacology; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Integrated Traditional and Western Medicine 2005;25(1):42-44
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the pharmacologic mechanism of gardenin in treating cerebral ischemia, by studying its effect on gene expression profile in brain of rats with focal cerebral ischemia (FCI).
METHODSTotal RNAs were isolated from rats with FCI and those treated with gardenin. The mRNAs were reversely transcribed to cDNA with incorporation of fluorescent Cy5- or Cy3-dUTP to prepare hybridization probes. The PCR products of 4096 genes were spotted on the chip after a serial treatment. The mixed probes were hybridized to the cDNA microarray. Axon Genepix 4000B and GenePixPro 3.0 software were used to scan and analyze the fluorescent signals.
RESULTSIn the group treated with gardenin, there were 70 genes had expression profiles different to that in the model group in the focal cerebral ischemic brain tissue, in which 68 were up-regulated and 2 down-regulated.
CONCLUSIONGardenin has regulatory effect on the gene expression in rats with focal cerebral ischemia, which elucidates part of the pharmacologic mechanism of Qingkailing in molecular level.
