Study on the differentially expressed genes in bone tissue of ovariectomized rats after intervention with Chinese herbs.
- Author:
Yu-ying MA
1
;
Xin-min ZHANG
;
Shi-feng LI
;
Yi-feng GU
;
Wei-hua CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone and Bones; metabolism; Drugs, Chinese Herbal; pharmacology; Female; Gene Expression Profiling; Gene Expression Regulation; drug effects; Osteoporosis; drug therapy; genetics; metabolism; Ovariectomy; Polymerase Chain Reaction; methods; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Integrated Traditional and Western Medicine 2005;25(2):138-142
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the therapeutic effect and mechanism of jiangu granule (JGG) on postmenopausal osteoporosis.
METHODSDifferential display reverse transcription PCR (DDRT-PCR) was used to detect the differentially expressed genes in bone tissues associated with therapeutic effect of JGG.
RESULTSTen differentially expressed gene fragments were found, 9 of them, after cloning, sequencing and BLAST searching, were proved to be matched with highly homologous sequences in Genebank. Among them, two were known proteins: hyaluronan-mediated motility receptor (RHAMM) and ATPase, Na+, K+ transporting beta 3 polypeptide (ATP1b3). It was confirmed by semi-quantitative RT-PCR that these two gene fragments could be down- and up-regulated by JGG respectively.
CONCLUSIONThe therapeutic effect of JGG in treating postmenopausal osteoporosis might be related with its regulation on the two kinds of protein, RHAMM and ATP1b3.
