Effect of puerarin and ligustrazine on cultured hippocampal neurons injury induced by Abeta25-35.
- Author:
Yu WANG
1
;
Hai-Tong WAN
;
Wei-Min YAN
;
Jie-Hong YANG
;
Ying GUO
;
Yuan-Jiang PAN
;
Jin HAN
;
Qin YU
Author Information
- Publication Type:Journal Article
- MeSH: Amyloid beta-Peptides; pharmacology; Animals; Cells, Cultured; Hippocampus; cytology; Isoflavones; pharmacology; Neurons; drug effects; Pyrazines; pharmacology; Rats
- From: China Journal of Chinese Materia Medica 2008;33(4):424-427
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes of MDA, SOD, LDH of cultured hippocampal neurons injury induced by amyloid-beta protein (Abeta 25-35) and the protective effect of puerarin and ligustrazine.
METHODPrimary hippocampal neurons were cultured and induced by Abeta 25-35. The concentrations of MDA, SOD and LDH in cultured hippocampal neurons were measured after exposed to Abeta 25-35, puerarin and ligustrazine.
RESULTThe Alzheimer disease (AD) model was successfully established in cultured hippocampal neurons. AD group has remarkably increased MDA and LDH level, and decreased SOD level, Piracetan group and combined application group of have remarkably decreased MDA and LDH level and increased SOD level, compared with AD group (P < 0.01). Ligustrazine together with puerarin group has remarkably decreased MDA and LDH level and increased SOD level, compared with ligustrazine group and puerarin group (P < 0.05).
CONCLUSIONAbeta 25-35 can induce cultured hippocampal neurons injury, combined application of ligustrazine, and puerarin can alleviate the injury.
