Study on excretion of pseudo-ginsenoside GQ.
- Author:
Chun-Fang ZHAO
1
;
Jin-Ping LIU
;
Yan ZHAO
;
Ping-Ya LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bile; metabolism; Chromatography, High Pressure Liquid; Feces; Female; Ginsenosides; administration & dosage; metabolism; pharmacokinetics; urine; Male; Rats; Rats, Sprague-Dawley; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction
- From: China Journal of Chinese Materia Medica 2008;33(4):432-435
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the pseudo-ginsenoside GQ (PGQ) in rat bile, feces and urine, and to study on the excretion of pseudo-ginsenoside GQ.
METHODReverse phase high-performance liquid chromatography (RP-HPLC) method with an evaporative light-scattering detector (ELSD) was performed on Diamonsil C18 column (4.6 mm x 250 mm, 5 microm), and the mobile phase was consisted of methanol-water (24: 7) with flow rate of 1.0 mL x min(-1). ELSD parameters were set as follows: nitrogen gas pressure 3.0 bar, drift tube temperature 50 degrees C.
RESULTThe method fulfilled all the standard requirements of precision, accuracy and linearity. The main way of excretion of PGQ in rat administrated through sublingual vein was at the bile. The bile excretion ratio of PGQ was 41.60%, and feces excretion ratio was 9.97%. Only trace amount of PGQ was excreted in urine.
CONCLUSIONAlmost all unchanged PGQ was excreted in bile, feces and urine.
