Effect of Capparis spinosa on fibroblast proliferation and type I collagen production in progressive systemic sclerosis.
- Author:
Yue-Lan CAO
1
;
Xin LI
;
Min ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Capparis; chemistry; Cell Proliferation; drug effects; Cells, Cultured; Collagen Type I; metabolism; Drugs, Chinese Herbal; pharmacology; Fibroblasts; cytology; drug effects; Humans; Scleroderma, Diffuse; drug therapy; metabolism; pathology
- From: China Journal of Chinese Materia Medica 2008;33(5):560-563
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of ethanolic extract from Capparis spinosa (ECS) on the fibroblast proliferation and type I collagen production in normal and progressive systemic sclerosis (PSS).
METHODCellular activity was determined by the MTT method. Apoptosis was detected by flow cytometry analysis of Annexin V-stained cells. The expression levels of type I collagen messenger RNA and protein were analyzed by RT-PCR and western blot analysis.
RESULTECS could significantly inhibit the proliferation of fibroblast and reduced the expression of alpha2 (I) collagen mRNA and type I collagen protein in PSS in a dose-and time-dependent manner. ECS did not affect the proliferation of fibroblast and expression of type I collagen mRNA and protein in normal human. ECS could counteract the harmful effects on fibroblast by H2O2.
CONCLUSIONECS can effectively inhibit the fibroblast proliferation and type I collagen production in PSS.
