Determination of content of saikoside in Bupleurum Chinense from different sources.
- Author:
Yuan-Yuan LI
1
;
Xue-Mei QIN
;
Yu-Qing WANG
;
Jian-Ying YUE
;
Jian-Ping HAO
Author Information
- Publication Type:Journal Article
- MeSH: Bupleurum; chemistry; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; chemistry; Oleanolic Acid; analogs & derivatives; analysis; chemistry; Reproducibility of Results; Saponins; analysis; chemistry
- From: China Journal of Chinese Materia Medica 2008;33(3):237-240
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the contents of saikosaponin a, d and saikosaponins in Radix Bupleuri from different and assess their quality.
METHODSaikosaponin a and d were used as the chemical reference substance to establish RP-HPLC and visible-spectrophotometry methods for determination of contents of saikosaponin a, d and saikosaponins.
RESULTThe quality of B. chinense was better than that of B. scozonerifolium and B smishii; The contents of a, saikosaponin d and total saikosaponin in B. chinense were 0.100%-0.330%, 0.200%-0.400% and 1.00%-2.00% , respectively.
CONCLUSIONIt is to suggest that the minimal contents requirements of saikosaponin a, d and saikosaponins in B. chinense should be set at 0. 100% , 0.200% and 1.00% , respectively; the best suitable planting areas are Taigu in the middle of Shanxi province, Ruicheng, Pinglu and Wanrong in the south of Shanxi province, and Longxi, Lixian in Gansu province.
