RP-HPLC determination of betulinic acid in Callicarpa macrophylla.
- Author:
Ping PAN
1
;
Ling-Yun JIA
;
Qi-Shi SUN
Author Information
- Publication Type:Journal Article
- MeSH: Calibration; Callicarpa; chemistry; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; chemistry; Linear Models; Quality Control; Reproducibility of Results; Sensitivity and Specificity; Triterpenes; analysis; isolation & purification
- From: China Journal of Chinese Materia Medica 2008;33(7):753-755
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an RP-HPLC method for determination of betulinic acid in Callicarpa macrophylla, a commonly used herbal in Yunnan.
METHODA Kromasil-C18 column (4.6 mm x 200 mm, 5 microm) and a mobile phase consisted of acetonitrile-0.1% phosphoric acid (63: 37) were used. The flow rate was 1.0 mL x min(-1) and the UV detector wavelength was 205 nm.
RESULTBetulinic acid was well separated from other compounds in C. macrophylla. The content of betulinic acid in C. macrophylla from different origins showed apparent differences, the content of betulinic acid in C. macrophylla from Yunnan was the highest. The calibration curve was linear in the range of 0.016 6-0.332 mg x mL(-1) of betulinic acid with correlation coefficient 0.999 8. The average recovery of betulinic acid was 98.5%.
CONCLUSIONThe method is simple, accurate and reproducible, and is suitable for the quality control of C. macrophylla.
