Determination of vetatramine in Veratrum nigrum by HPLC-ELSD.
- Author:
Li-Shu WANG
1
;
Da-Qing ZHAO
;
Hua-Ming TAO
;
Yong-Hong LIU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; chemistry; Linear Models; Reproducibility of Results; Sensitivity and Specificity; Veratrum; chemistry; Veratrum Alkaloids; analysis; isolation & purification
- From: China Journal of Chinese Materia Medica 2008;33(7):791-792
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC-ELSD method for determination of vetatramine. in Veratrum nigrum.
METHODThe analy fical column was Shim-pack ODS - C18 (4.6 mm x 250 mm, 4 microm) column, the mobile phase was acetonitrile-water (containing 0.1% triethylamine) (50:50), at a flow rate of 0.8 mL x min(-1). The temperature of drift tube was 90 degrees C and the gas flow was at the rate of 2.5 L x min(-1).
RESULTThe calibration curve was linear in the range of 0.36-3.6 microg (r = 0.999 8). The average recovery was 100.9% (RSD 2.3%, n = 6). The contents of veratramine in Veratrum nigrum. from the ten different sources were determined.
CONCLUSIONThe method may be used as a accurate and reproducible way to determine the content of veratramine in V. nigrum.
