Effect of emodin on hepatocellular apoptosis following orthotopic liver transplantation in rats.
- Author:
Sheng-Zhang LIN
1
;
Xian-Chou XU
;
He JING
;
Xiao YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Emodin; pharmacology; Graft Rejection; Hepatocytes; cytology; drug effects; immunology; Liver Transplantation; Male; Rats; Rats, Inbred Lew
- From: China Journal of Chinese Materia Medica 2008;33(7):809-812
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effect of emodin on hepatocellular apoptosis following orthotopic liver transplantation (OLT) in rats.
METHODThe LEW --> BN OLT models were established. A total of 24 rats were divided randomly and equally into 4 groups. Group A was treated with normal saline at dose of 0.5 mL x d(-1) intraperitoneally from 1st day to 8 th day after operation. Group B, CsA at dose of 10.0 mg x kg(-1) x d(-1). Group C, emodin at dose of 50.0 mg x kg(-1) x d(-1). Group D, CsA at dose of 10.0 mg x kg(-1) x d(-1) and emodin at dose of 50.0 mg x kg(-1) x d(-1). Fifteen days after operation, rejection active index (RAI) and hepatocellular apoptosis index (AI) was confirmed after observing the pathologic change of transplanted liver in recipients.
RESULTRespectively, the RAI of group A, B, C, D was 7.67 +/- 0.98, 5.17 +/- 0.40, 5.83 +/- 0.75, 3.83 +/- 0.75 and the AI of group A, B, C, D was 35.83 +/- 2.320, 15.83 +/- 1.33, 16.50 +/- 2.35, 11.50 +/- 1.05. The RAI and AI of group B, C, D was significantly lower than group A (P < 0.01) and group D was significantly lower than group B, C too (P < 0.05). There was no significant distinction between group B and C in RAI and AI.
CONCLUSIONEmodin has the effect of reduce the hepatocellular apoptosis following OLT in rats and the effect can stronger by CsA.
