Inhibitory effects of sinapine on activity of acetylcholinesterase in cerebral homogenate and blood serum of rats.
- Author:
Ling HE
1
;
Hai-Tao LI
;
Sheng-Wei GUO
;
Li-Fang LIU
;
Jia-Bin QIU
;
Fu LI
;
Bao-Chang CAI
Author Information
- Publication Type:Journal Article
- MeSH: Acetylcholinesterase; blood; metabolism; Alzheimer Disease; drug therapy; prevention & control; Animals; Brain; cytology; enzymology; Choline; analogs & derivatives; pharmacology; therapeutic use; Cholinesterase Inhibitors; pharmacology; therapeutic use; Rats
- From: China Journal of Chinese Materia Medica 2008;33(7):813-815
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe present study investigated the inhibitory effects of Chinese herb component sinapine on activity of acetylcholinesterase (AChE) in cerebral homogenate and blood serum of rats.
METHODAChE was prepared from cerebral homogenate and blood serum of rats, respectively. Acetylcholinesterase activity assay kit and Chromatometry were used to detect the AChE activity.
RESULTSinapine significantly inhibited AChE activity in vitro, with more effective on cerebral homogenate (IC50 3.66 micromol x L(-1)) than blood serum (IC50 22.1 micromol x L(-1)).
CONCLUSIONSinapine could significantly inhibit the cerebral AChE activity and may be a promising drug used for prevention and cure of Alzheimer's disease as a cholinesterase inhibitor.
