Correlations of podocyte injury with glucose regulated protein 78 expression and proteinuria in patients with diabetic nephropathy.
- Author:
Ying-jiu LIU
1
;
Yu-bing WEN
;
Jian-ling TAO
;
Jin-hong LI
;
Ying SU
;
Wei YE
;
Hang LI
;
Xue-mei LI
;
Xue-wang LI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Diabetic Nephropathies; complications; metabolism; pathology; Female; Heat-Shock Proteins; metabolism; Humans; Male; Middle Aged; Podocytes; pathology; Proteinuria; etiology
- From: Acta Academiae Medicinae Sinicae 2012;34(4):359-363
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the podocyte injury in patients with diabetic nephropathy (DN) and analyze its relationship with glucose regulated protein 78 (GRP78) and proteinuria.
METHODSThe clinical data of 48 patients diagnosed as DN by renal biopsy were reviewed. All patients were divided into two groups according to proteinuria (>3.5 g/d, n=31 and 3.5 g/d, n=17). The density of podocytes was illustrated by immunohistochemistry staining of Wilms tumor-1 (WT-1), and the immunofluorescence double-staining results of synaptopodin and GRP78 in podocytes were detected.
RESULTSThe podocyte dentistry of urine protein > 3.5 g/d group was significantly lower than that of urine protein>3.5 g/d group urine protein<3.5 g/d group(P=0.003), and it was negatively correlated with proteinuria (P=0.005). The expressions of synaptopodin and GRP78 in podocytes were also negatively correlated with proteinuria (P=0.004 and P=0.001).
CONCLUSIONThe podocyte injury is aggravated with increased proteinuria in DN patients, along with the decrease of the adaptive ability of endoplasmic reticulum to stress.
