Expression of hypoxia-inducible factor 1α in osteosarcoma and its value in predicting chemosensitivity.
- Author:
Yong CHEN
1
;
Chun-meng WANG
;
Ying-qiang SHI
;
Yun YANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Antineoplastic Combined Chemotherapy Protocols; therapeutic use; Bone Neoplasms; drug therapy; metabolism; pathology; Chemotherapy, Adjuvant; Child; Cyclooxygenase 2; metabolism; DNA-(Apurinic or Apyrimidinic Site) Lyase; metabolism; Disease-Free Survival; Female; Follow-Up Studies; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; metabolism; Male; Middle Aged; Neoadjuvant Therapy; Neoplasm Staging; Osteosarcoma; drug therapy; metabolism; pathology; Vascular Endothelial Growth Factor A; metabolism; Young Adult
- From: Chinese Journal of Oncology 2012;34(12):899-904
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the prognostic values of HIF-1α, APE1, VEGF, and COX-2 protein expressions and their predictive value of tumor necrosis rate and prognosis in osteosarcoma, as well as their interrelationships.
METHODSFormalin-fixed paraffin-embedded tissue samples were obtained from patients with osteosarcoma. Immunohistochemical assay was performed in pre-chemotherapy samples to determine the HIF-1α, VEGF, APE1, and COX-2 protein expression levels. Hematoxylin-eosin staining was used in post-operative samples to determine the tumor necrosis rate. Univariate and multivariate analyses were used to assess the impact of protein expression on prognosis.
RESULTSTumor tissues were obtained from 49 patients. Their median follow up was 29 months. HIF-1α was significantly correlated to every protein we tested: VEGF (P = 0.032), APE1 (P < 0.001), and COX-2 (P < 0.001). HIF-1α protein expression had a significant impact on disease free survival (P = 0.006). Expression of HIF-1α had a sensitivity of 64.7% and a specificity of 71.9% for poor pathological response (< 90% of tumor necrosis) versus good pathological response to chemotherapy (≥ 90% necrosis).
CONCLUSIONExpression of HIF-1α is a predictor of tumor response to neoadjuvant chemotherapy and outcome in osteosarcoma and is correlated with VEGF, APE1, and COX-2 expression.
