Inhibitory effect of silencing of HMGB1 gene expression on the invasive and metastatic abilities of MGC-803 gastric cancer cells.
- Author:
Zeng-jun LI
1
;
Bao SONG
;
Jie LIU
;
Jian-jun HAN
;
Chuan-xi WANG
;
Yun-xia ZHU
;
Zhong-fa XU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Adhesion; Cell Line, Tumor; Cell Movement; Down-Regulation; Gene Expression Regulation, Neoplastic; HMGB1 Protein; genetics; metabolism; Humans; Matrix Metalloproteinase 9; genetics; metabolism; NF-kappa B; genetics; metabolism; Neoplasm Invasiveness; Neoplasm Metastasis; RNA, Messenger; metabolism; RNA, Small Interfering; genetics; Stomach Neoplasms; metabolism; pathology; Transfection
- From: Chinese Journal of Oncology 2013;35(4):244-248
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of high mobility group box-1 (high mobility group box B 1, HMGB1) on the invasive and metastatic abilities of gastric cancer cell line MGC-803 and analyze the possible mechanisms.
METHODSHMGB1 gene targeting siRNA was designed and synthesized, and HMGB1 siRNA oligonucleotides were transfected into the MGC-803 cells with Lipofectamine 2000. The invasive and migratory abilities were detected by transwell assay and scratch assay. The Matrigel matrix glue adhesive ability of MGC-803 cells was evaluated by MTT assay. NF-κB activity was detected by electrophoretic mobility shift assay. The mRNA and protein levels of HMGB1 and MMP-9 were determined by RT-PCR and Western blot, respectively.
RESULTSThe siRNA down-regulated the levels of HMGB1 mRNA and protein. Compared with that of the control group, the number of invasive (142.7 ± 3.4 /view vs. 303.5 ± 4.3/view) and migratory (293.7 ± 4.4/view vs. 445.5 ± 5.6/view) cells was significantly increased (P < 0.05) and the adhesive ability of MGC-803 cells to Matrigel was significantly elevated (33.4 ± 0.03% vs. 57.4 ± 4.2%, P < 0.05). In addition, silencing of HMGB1 gene significantly inhibited the activity of NF-κB and the relative expression folds of mRNA (0.2 ± 0.1 vs. 1.4 ± 0.4, P < 0.05)and protein (0.4 ± 0.1 vs. 2.3 ± 0.7, P < 0.05) of MMP-9.
CONCLUSIONSilencing of HMGB1 can effectively inhibit the invasion and migration of gastric cancer cells and this effect of HMGB1 may be partly due to its regulation of NF-κB and MMP-9 expressions.
